Exogenous application of silicon and selenium improves the tolerance of tomato plants to calcium nitrate stress.

Silicon (Si) and selenium (Se) can improve the tolerance of plants to NaCl-induced salt stress. However, few studies are available on their regulatory effects on plants' tolerance to calcium nitrate stress, which often occurs in protected facilities, causing secondary soil salinization. In this study, we report the effects of Si (6 mM) and Se (20 µM) applied separately or in combination on the growth, photosynthesis, oxidative damage, and nitrogen metabolism of tomato plants, as well as fruit quality under calcium nitrate stress. The results showed that applications of Si or Se alone or in combination improved the plant growth and photosynthetic performance and reduced oxidative damage of the stressed plants. Applications of Si and Se did not decrease the calcium accumulation in leaves of the stressed plants. Under calcium nitrate stress, the concentrations of NO3-, NO2- and NH4+ in leaves were significantly increased, while the activities of nitrogen assimilation-related enzymes (including nitrate reductase, nitrite reductase, glutamine synthase, glutamine-2-oxoglutarate aminotransferase and glutamate dehydrogenase) were decreased. Applications of Si and Se, especially their combined treatment, decreased the NO3-, NO2-, and NH4+ concentrations and enhanced the activities of nitrogen assimilation-related enzymes in the stressed plants. Applied Si and Se also decreased the nitrate and titratable acid concentrations and increased vitamin levels in tomato fruits under calcium nitrate stress. It is suggested that Si and Se improved the tomato plant growth and fruit quality under calcium nitrate stress by alleviating oxidative damage and promoting both photosynthesis and nitrogen assimilation.

Silicon improves the growth of cucumber under excess nitrate stress by enhancing nitrogen assimilation and chlorophyll synthesis.

Silicon (Si) can increase salt tolerance of plants, and previous studies have focused on NaCl stress; whereas in protected facilities, nitrate (but not NaCl) accumulation is one of the major causes of secondary soil salinization. However, information on Si's effect on plant growth under nitrate stress is very limited, and the underlying mechanism is unknown. Here, we investigated Si's effect on plant growth, nitrogen assimilation and chlorophyll synthesis in cucumber. Cucumber seedlings ('Jinyou 1') were subjected to 200 mM nitrate stress without or with addition of 2 mM Si. The results showed that root application, but not foliar application of Si, could improve cucumber growth under nitrate stress. Root addition of Si increased photosynthetic rate and decreased oxidative damage of stressed plants. Under nitrate stress, Si addition decreased the accumulation of nitrate, nitrite and ammonium, and promoted the activities of nitrate reductase, nitrite reductase, glutamine synthase, glutamine-2-oxoglutarate aminotransferase and glutamate dehydrogenase in leaves. The concentrations of glutamic acid, 5-aminolevulinic acid, porphobilinogen and uroporphyrinogen Ⅲ were increased under nitrate stress, while these were decreased by added Si. Added Si increased the levels of chlorophyll and its precursors (protoporphyrin Ⅸ, Mg-protoporphyrin Ⅸ and protochlorophyllide), and expressions of genes encoding enzymes in chlorophyll synthesis (CHLH, POR and CAO) under nitrate stress. These results suggest that Si could improve cucumber growth under nitrate stress by enhancing nitrogen assimilation and chlorophyll synthesis, and imply an application of Si fertilizer in solving secondary soil salinization in protected facilities.